I. Dweikat et al., IDENTIFICATION OF RAPD MARKERS FOR 11 HESSIAN FLY RESISTANCE GENES INWHEAT, Theoretical and Applied Genetics, 94(3-4), 1997, pp. 419-423
The pyramiding of genes that confer race- or biotype-specfic resistanc
e has become increasingly attractive as a breeding strategy now that D
NA-based marker-assisted selection is feasible. Our objective here was
to identify DNA markers closely linked to genes in wheat (Triticum ae
stivum L.) that condition resistance to Hessian fly [Mayetiola destruc
tor (Say)]. We used a set of near-isogenic wheat lines, each carrying
a resistance gene at 1 of 11 loci (H3, H5, H6, H9, H10, H11, H12, H13,
H14, H16 or H17) and developed by backcrossing to the Hessian fly-sus
ceptible wheat cultivar 'Newton'. Using genomic DNA of these 11 lines
and 'Newton', we have identified 18 randomly amplified polymorphic DNA
(RAPD) markers linked to the 11 resistance genes. Seven of these mark
ers were identified by denaturing gradient gel electrophoresis and the
others by agarose gel electrophoresis. We confirmed linkage to the He
ssian fly resistance loci by cosegregation analysis in Fz populations
of 50-120 plants for each different gene. Several of the DNA markers w
ere used to determine the presence/absence of specific Hessian fly res
istance genes in resistant wheat lines that have 1 or possibly multipl
e genes for resistance. The use of RAPD markers presents a valuable st
rategy for selection of single and combined Hessian fly resistance gen
es in wheat improvement.