ULTRASOUND PERMEABILIZES CHO CELLS FOR THE ENDONUCLEASES ALUI AND BENZON NUCLEASE

Ultrasound permeabilizes Chinese hamster ovary (CHO) cells for the end onucleases AluI and benzon nuclease which leads to the induction of ch romosomal aberrations by these enzymes. A few aberrant cells were obse rved when trypsinized cells or adherent cells were exposed to the enzy mes in the absence of ultrasound. Our data show that sonication can he used to introduce endonucleases into CHO cells. We further demonstrat e that few cells can internalize endonucleases without previous permea bilization.