RESOLUTION OF NATURAL HATCHING FACTORS FOR GOLDEN POTATO CYST-NEMATODE, GLOBODERA-ROSTOCHIENSIS

The hatching responses of Globodera rostochiensis (golden potato cyst nematode) to purified and partially-purified preparations of natural ( including the potato glycoalkaloids solanine and alpha-chaconine) and artificial hatching factors (HFs) were bimodal. At least 10 HFs, mostl y anionic, were resolved from potato root leachate by a combination of gel permeation and ion-exchange chromatography. Whereas potato roots were the principal source of HFs, haulm leachate also contained such c hemicals. Root leachate from aseptically-grown potato plants lacked se veral HFs which were present in conventionally-produced leachate.