FRACTIONATION AND CHARACTERIZATION OF BOAR SEMINAL PLASMA SPERMADHESIN PSP-II GLYCOFORMS REVEAL THE PRESENCE OF UNCOMMON N-ACETYLGALACTOSAMINE CONTAINING N-LINKED OLIGOSACCHARIDES

Lectin mapping, carbohydrate analysis and electrospray mass spectromet ry of boar seminal plasma PSP-II glycoforms show that its single N-gly cosylation site displays a repertoire of carbohydrate structures consi sting of the basic pentasaccharide core Man alpha 1-6 [Man alpha 1-3]M an beta 1-4GlcNAc beta 1-4GlcNAc with a fucosyl residue alpha 1-6-link ed to the innermost N-acetylglucosamine residue. Other glycoforms disp lay fucosylated hybrid-type or monoantennary complex-type chains, some of which contain alpha 2-6-linked sialic acid. N-acetylgalactosamine, possibly in Gal beta 1-3GalNAc sequence, is present in most of the PS P-II glycoforms.