ENGINEERING THERMOLYSIN-LIKE PROTEASES WHOSE STABILITY IS LARGELY INDEPENDENT OF CALCIUM

Authors
VELTMAN OR VRIEND G VANDENBURG B HARDY F VENEMA G EIJSINK VGH
Citation
Or. Veltman et al., ENGINEERING THERMOLYSIN-LIKE PROTEASES WHOSE STABILITY IS LARGELY INDEPENDENT OF CALCIUM, FEBS letters, 405(2), 1997, pp. 241-244
Citations number
19
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
405
Issue
2
Year of publication
1997
Pages
241 - 244
Database
ISI
SICI code
0014-5793(1997)405:2<241:ETPWSI>2.0.ZU;2-A
Abstract
Thermal stability of the thermolysin-like protease produced by Bacillu s stearothermophilus (TLP-ste) is highly dependent on calcium at conce ntrations in the millimolar range. We describe the rational design and production of a fully active TLP-ste variant whose stability is only slightly dependent on calcium concentration. (C) 1997 Federation of Eu ropean Biochemical Societies.