K. Langworthy et al., AMINES AND PEPTIDES IN THE BRAIN OF THE AMERICAN LOBSTER - IMMUNOCYTOCHEMICAL LOCALIZATION PATTERNS AND IMPLICATIONS FOR BRAIN-FUNCTION, Cell and tissue research, 288(1), 1997, pp. 191-206
The distributions of serotonin- (5HT-), substance P- (SP-), small card
ioactive peptide(b-) (SCPb-), and histamine- (HA-) like immunoreactivi
ties were examined in the adult lobster supraesophageal ganglion. Vibr
atome sections were labeled using avidin-biotin-peroxidase immunocytoc
hemical methods. The localization patterns for each substance were ass
essed in 21 regions within the median protocerebrum deutocerebrum and
tritocerebrum. Each immunoreactivity has a unique distribution within
the brain; however, most regions are immunoreactive for more than one
neurotransmitter. Of particular interest are SP-immunoreactive protoce
rebral neurons that contact olfactory projection neurons and appear ho
mologous to those found in other crustaceans. Regional differences in
immunolabeling within the deutocerebral olfactory and accessory lobes
suggest that specific areas within individual olfactory lobe glomeruli
serve distinct functions in olfactory processing, and that subpopulat
ions of accessory lobe glomeruli are innervated by different groups Of
neurons. This detailed comparison of the labeling patterns also has a
llowed us to define the anatomical connectivity between several cell b
ody clusters, fiber tracts, and neuropil areas in the lobster brain.