HIGH-FREQUENCY ADVENTITIOUS PLANT-REGENERATION FROM RADICLE EXPLANTS OF AEGLE MARMELOS CORR

A procedure was developed which allows high frequency adventitious sho ot regeneration from radicle tissues of Aegle marmelos. Adventitious b uds were initiated on Murashige & Skoog's (MS) medium containing vario us combinations of benzyladenine (BA) and naphthaleneacetic acid (NAA) . The medium containing 1.0 mg/l BA and 0.2 mg/l NAA produced highest number of shoots per explant with maximum frequency of regeneration. S hoots were elongated by transferring explants with shoot buds to a med ium with a low concentration (0.1 mg/l) of BA. The shoots grown in med ium containing 25 mg/l indolebutyric acid (IBA) for one week, when tra nsferred to basal medium produced adventitious roots. Maximum rooting (80%) with 3-6 roots per shoot was achieved.