B. Gawronskaszklarz et al., N-ACETYLATION AND HYDROXYLATION POLYMORPHISMS IN TYPE-II DIABETICS WITH MICROVASCULAR DISTURBANCES, European Journal of Clinical Pharmacology, 51(6), 1997, pp. 431-435
The N-acetylation and hydroxylation (CYP2D6) genetic polymorphisms wer
e assessed in 43 healthy subjects and in 84 type II (non-insulin-depen
dent) diabetics. The proportions of slow and fast acetylators as well
as poor and extensive metabolisers in a group of diabetics suffering f
rom microvascular disturbances (nephropathy, retinopathy and neuropath
y) were compared with the ocntrol group and with diabetics without suc
h ocmplications. Sulphadimidine was used as a probe for polymorphic ac
etylation and debrisoqine for CYP2D6. Debrisoquine and its 4-OH metabo
lite were assayed by means of HPLC, and sulphadimidine using a modifie
d Bratton-Marshall proceedure. The frequency of the slow phenotype (63
%) was significantly higher in diabetics with microvascular disturbanc
es than in patiens without diabetic complications (P < 0.005). In pati
ents with type II diabetes (84), only the extensive phenotype of hydro
xylation was observed.