USE OF BENZOYL HYDRAZINE REAGENT FOR MONOSACCHARIDE DETERMINATION BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS

Benzoyl hydrazine derivatives of reducing carbohydrates were analyzed by high performance capillary electrophoresis with on-tube ultraviolet detection. The derivatives of 9 reducing monosaccharides were complet ely separated in ca. 30 min. using a capillary tube (50 mu m i.d., 62 cm) and 200 mM berate buffer, pH 10.8, as carrier. On-column UV detect ion at 220 nm was able to achieve sensitive detection down to 30 simil ar to 40 femtomole levels. The experimental parameters for optimum pre column are discussed along with derivatization and separation. It was demonstrated that this method allows quantification of reducing carboh ydrates with high accuracy and reproducibility.