HIGH-LEVEL EXPRESSION OF THE IL-1 RECEPTOR-RELATED T1 RECEPTOR IN INSECT CELLS

Baculovirus-dependent expression of membrane-associated and secreted, heavily modified proteins in insect cells often results in low yields. To optimize expression of T1, a heavily glycosylated receptor related to Interleukin-1 receptor type I, deletion mutants comprising either the heavily glycosylated extracellular domain or the isolated transmem brane and cytoplasmic portions of the T1 receptor were expressed from recombinant baculoviruses, As shown here, the use of a Baculovirus-der ived leader sequence (GP67) in combination with an insect cell line wi th increased secretory capacity resulted in yields of several mg per l iter (10(9) cells) of culture of both purified secretory T1 glycoprote in and membrane-associated T1 receptor. (C) 1995 Academic Press, Inc.