OPTIMIZATION OF EXPERIMENTAL CONDITIONS O F PLANAR CHROMATOGRAPHY WITH MULTIVARIATE-ANALYSIS .2. OPTIMIZATION OF SEPARATION OF QUINOLIZIDINIC ALKALOIDS BY ASSOCIATION CRITERIA BETWEEN SORBENTS AND ELUENTS

From 97 chromatograms concerning 87 quinolizidinic Fabaceae alkaloids on tile one hand and three standard chromatographic conditions on the other hand about hRf of 38 quinolizidinic alkaloids, we used mathemati cal statistical treatments to test the possibility to optimize chromat ographic separation methods; to find empirical rides which govern asso ciation between sorbents/ eluents/acid or basic additive; and to acces s to chromatographic typologies of chemical structure possessing the s ame molecular skeleton The results permit us to consider multidimensio nal relationships between structure and chemical and physicochemical p roperties. In this study we have used various mathematical statistic m ethods such as non-hierarchic classification (minimal spanning tree) h ierarchic ascending classification (dendrogram) and correspondence ana lysis. This approach maybe generalized to a more important homogeneous chromatographic information corpus with a viewpoint to determine its statistical validity and limits.