SEPARATION OF MAJOR POLAR LIPIDS IN PECTEN-MAXIMUS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND SUBSEQUENT DETERMINATION OF THEIR FATTY-ACIDS USING GAS-CHROMATOGRAPHY

Authors
SOUDANT P MARTY Y MOAL J SAMAIN JF
Citation
P. Soudant et al., SEPARATION OF MAJOR POLAR LIPIDS IN PECTEN-MAXIMUS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND SUBSEQUENT DETERMINATION OF THEIR FATTY-ACIDS USING GAS-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 673(1), 1995, pp. 15-26
Citations number
40
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
673
Issue
1
Year of publication
1995
Pages
15 - 26
Database
ISI
SICI code
Abstract
An easy method for the separation of major polar lipid classes by HPLC is described. Maximum resolution was achieved by an automated combina tion of a silica gel column and a diol column. Polar lipid analysis of the larvae and gonads of Pecten maximus showed the presence of a part icular glycolipid especially rich in 22:6(n - 3) and the predominance of 20:4(n - 6) in the phosphatidylinositol. The phosphatidylcholine an d phosphatidylethanolamine (diacyl form + alkenylacyl) were the major fractions. The plasmalogen form (25% in larvae, 34% in gonads) was ess entially composed of polyunsatured fatty acids of 20 and 22 carbons in the sn-2 position.