DETERMINATION OF THE ENANTIOMERIC PURITY OF 5,6-DIHYDROXY-2-AMINOTETRALIN BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS WITH CROWN-ETHER ASCHIRAL SELECTOR

5,6-Dihydroxy-2-aminotetralin was resolved into its enantiomers by hig h-performance capillary electrophoresis. The separation was performed with a background electrolyte containing 18-crown-6 tetracarboxylic ac id as chiral selector. The chiral recognition is based on a primary in teraction between the hydrogens of the amino group and the oxygens of the crown ether and a secondary interaction between the catechol and t he carboxylates of the crown ether. The resolution factor was 1.42 and the detection limit of the enantiomer present as impurity was 0.3%. D ata on the optimization and the validation of the assay are also repor ted.