CDNA CLONING OF MOUSE FERREDOXIN REDUCTASE FROM KIDNEY

A cDNA encoding ferredoxin reductase has been isolated from a mouse ki dney cDNA library using human ferredoxin reductase cDNA as a probe. Mo use ferredoxin reductase coded for 494 amino acid residues. The mouse mature enzyme which comprises 460 amino acid residues shared 87.8-89.1 % amino acid identities with the bovine and human enzyme. Northern blo t analysis showed that ferredoxin reductase mRNA was expressed in the adrenal, testis and ovary and to a lesser extent in the liver and kidn ey. However, this mRNA in the adrenal cell line, Y-1 cell, was not ind uced by adenosine 3',5'-cyclic monophosphate (cAMP) in contrast with f erredoxin mRNA.