CLONING AND CHARACTERIZATION OF FULL-LENGTH CDNAS CODING FOR THE DNA TOPOISOMERASE-II-BETA FROM CHINESE-HAMSTER LUNG-CELLS SENSITIVE AND RESISTANT TO 9-OH-ELLIPTICINE

Authors
DEREUDDRE S FREY S DELAPORTE C JACQUEMINSABLON A
Citation
S. Dereuddre et al., CLONING AND CHARACTERIZATION OF FULL-LENGTH CDNAS CODING FOR THE DNA TOPOISOMERASE-II-BETA FROM CHINESE-HAMSTER LUNG-CELLS SENSITIVE AND RESISTANT TO 9-OH-ELLIPTICINE, Biochimica et biophysica acta, N. Gene structure and expression, 1264(2), 1995, pp. 178-182
Citations number
26
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1264
Issue
2
Year of publication
1995
Pages
178 - 182
Database
ISI
SICI code
0167-4781(1995)1264:2<178:CACOFC>2.0.ZU;2-#
Abstract
DNA topoisomerase II beta cDNAs from Chinese hamster lung cells sensit ive (DC-3F) and resistant to 9-OH-ellipticine (DC-3F/90-HE) were isola ted. In the sensitive cells, the sequence defines an open reading fram e of 4839 nucleotides, and extends over a 323 nucleotides untranslated region up to the putative polyadenylation site. The deduced amino aci d sequence predicts a protein with 1612 amino acids in length and a ca lculated molecular mass of approx. 182 kDa. The cDNAs from the resista nt cells only differs by one mutation at position 1710 which converts a Trp codon (TGG) to a stop codon (TGA). This mutation accounts for th e loss of DNA topoisomerase II beta in the 9-OH-ellipticine resistant cells.