INHIBITION OF SODIUM ARTESUNATE ON RAT ER YTHROCYTE-MEMBRANE NA-(-K-(+)-EXCHANGING ATPASE IN-VITRO())

AIM: To study the effects of sodium artesunate I(SA) on Na-(+)-K-(+)-e xchanging ATPase activity of rat erythrocyte membrane in vitro. METHOD S: SA of different concentrations (0, 0.5, 1, 5 and 10 mu mol . L(-1)) were added respectively in the reaction system. The . Na-(+)-K-(+)-ex changing ATPase activity was calculated by measuring the amount of ino rganic phosphate released in the reaction system. RESULTS: Along with the increase of SA concentration (0, 0.5, 1, 5 and 10 mu mol . L(-1)), the Na-(+)-K-(+)-exchanging ATPase activity decreased, the inhibitory rates were 15 %, 29 %, 46 %, and 75 %, respectively. Increasing the c oncentration of substrate ATP to 125, 250, 375 and 500 mu mol . L(-1) and determining the kinetics of enzyme. Making the Eadie-Hofstee kinet ic curve by linear-regression analysis. The result revealed that the i nhibitory effect of SA on the enzyme was competitive. CONCLUSION: SA a ffected the ion transfer of host erythrocyte membrane and membrane fun ction.