M. Leconiat et al., PARTIAL CHROMOSOME-21 AMPLIFICATION IN A CHILD WITH ACUTE LYMPHOBLASTIC-LEUKEMIA, Genes, chromosomes & cancer, 14(3), 1995, pp. 204-209
Monosomy 21 and metacentric markers corresponding in size to chromosom
es 8 to 12 were found as the only clonal chromosomal changes in a chil
d with acute lymphoblastic leukemia (ALL). Chromosome painting with a
whole chromosome 21-specific probe showed that the marker originated f
rom chromosome 21. Fluorescence in situ hybridization with yeast artif
icial chromosome (YAC) probes to chromosome 21 showed genomic amplific
ation with two, four, or more copies of the probed DNA sequences prese
nt an the marker. The most amplified regions of chromosome 21 were cen
tromeric and telomeric to the Down's syndrome region. This observation
supports the notion that amplification of only parts of chromosome 21
may be important in the leukemogenic process in spite of the high inc
idence of complete trisomy 21 in ALL. (C) 1995 Wiley-Liss, Inc.