CAPILLARY ELECTROPHORESIS IN ALLERGEN PREPARATION RESEARCH AND IN PRODUCTION CONTROL

A CE method has been developed for the analysis of wasp venoms and pol len extracts of Phleum pratense and Betula verrucosa. Various electrol yte systems can be used but the best separation and reproducibility ar e attained in 0.15 mol/l phosphoric acid, pH 1.8, UV photometric detec tion at 190 nm is sufficiently sensitive for determination of the poly peptides and glycoproteins contained in these materials (the detection limit for phospholipase A(2) is 0.4 ng). The allergens from wasp veno ms and pollen extracts yield characteristic electropherograms which ca n be used for control of the allergen production. Modification of capi llary wall with poly(ethylhydroxy methacrylate) causes a decrease in t he migration times and the separation efficiency is retained.