EFFECT OF ORGANIC-SOLVENTS ON THE SEPARATION OF BENZOIC-ACIDS BY CAPILLARY ELECTROPHORESIS

Citation
Yj. Lee et al., EFFECT OF ORGANIC-SOLVENTS ON THE SEPARATION OF BENZOIC-ACIDS BY CAPILLARY ELECTROPHORESIS, Analyst, 120(11), 1995, pp. 2689-2694
Citations number
24
Categorie Soggetti
Chemistry Analytical
Journal title
ISSN journal
00032654
Volume
120
Issue
11
Year of publication
1995
Pages
2689 - 2694
Database
ISI
SICI code
0003-2654(1995)120:11<2689:EOOOTS>2.0.ZU;2-0
Abstract
The effect of organic modifiers on the separation of a number of close ly related isomeric benzoic acids by capillary electrophoresis is desc ribed. It is shown that while a single modifier concentration cannot h elp resolve the entire electropherogram, organic modifiers do signific antly enhance the resolution of parts of the separation system by comp arison with 40 mmol l(-1) phosphate buffer. The effects on separation and retention times are discussed in terms of the effects on electroos motic flow and the electrophoretic mobilities of the charged solutes. The effects were found to be modifier specific, although the trends we re in the same direction (Le., decreasing electroosmotic flow with inc reased percentage of organic modifier). The major influence is the man ipulation of the electroosmotic mobility.