DNA EXTRACTION AND AMPLIFICATION FROM GIEMSA-STAINED BLOOD SMEARS

Authors
YOKOTA M TATSUMI N TSUDA I YANO I
Citation
M. Yokota et al., DNA EXTRACTION AND AMPLIFICATION FROM GIEMSA-STAINED BLOOD SMEARS, Journal of clinical laboratory analysis, 9(6), 1995, pp. 387-391
Citations number
23
Categorie Soggetti
Medical Laboratory Technology
Journal title
Journal of clinical laboratory analysis → ACNP
ISSN journal
08878013
Volume
9
Issue
6
Year of publication
1995
Pages
387 - 391
Database
ISI
SICI code
0887-8013(1995)9:6<387:DEAAFG>2.0.ZU;2-I
Abstract
DNA extraction was attempted from Giemsa-stained blood smears on glass slides that had been stored for several years. High molecular weight DNA bands were clearly visible after electrophoresis when DNA was extr acted from specimens stored up to 2 years. Specimens more than 4 years old demonstrated a smeared pattern, suggesting degeneration of the DN A, but they could be rescued by PCR amplification using primers of HLA -DQA1 genes. The recovery could be pursued even in 11-year-old specime ns. (C) 1995 Wiley-Liss, Inc.