BIOSYNTHESIS OF ANTITUMOR ANTIBIOTIC, CYTOGENIN

The biosynthesis of antitumor antibiotic cytogenin, 3-hydroxymethyl-6- methoxy-8-hydroxy-isocoumarin, was studied by feeding C-14- Or C-13-la beled compounds to culture of the producing organism, Streptoverticill ium eurocidicum MI43-37F11. C-14-Acetate and C-14-methionine were effi ciently incorporated into cytogenin as precursors. C-13 NMR studies pr oved that the carbon skeleton of cytogenin is derived from pentaketide intermediate due to head-to-tail condensation of five acetate units a nd methyl group of 6-OCH3 is derived from methionine. It was suggested that 3-hydroxymethyl and/or 6-methoxy group of cytogenin were metabol ized by hydroxylation and/or methylation from three intermediates.