METABOLIC-ACTIVATION OF 4 DRUGS IN THE EYE MOSAIC ASSAY MEASURING PRINCIPALLY MITOTIC RECOMBINATION IN DROSOPHILA-MELANOGASTER - DIFFERENCES IN STRAIN SUSCEPTIBILITY AND ROUTE OF EXPOSURE
R. Rodriguezarnaiz et Jh. Aranda, METABOLIC-ACTIVATION OF 4 DRUGS IN THE EYE MOSAIC ASSAY MEASURING PRINCIPALLY MITOTIC RECOMBINATION IN DROSOPHILA-MELANOGASTER - DIFFERENCES IN STRAIN SUSCEPTIBILITY AND ROUTE OF EXPOSURE, MUTATION RESEARCH, 305(2), 1994, pp. 157-163
One mycotoxin and three therapeutic drugs widely used in developing co
untries were examined for genotoxic activity by means of w/w + somatic
recombination assay. Streptozotocin (SZ), an antibiotic antineoplasti
c agent, gave a frequency of light spots almost one order of magnitude
higher than those obtained with the carcinogen mycotoxin sterigmatocy
stin (STC), the antiprotozoal and antimicrobial metronidazole (MNZ), a
nd the antifungal griseofulvin (GF). Thus the order of response was SZ
>STC>MNZ>GF. Chronic treatment turned out to be the better route of ex
posure for these genotoxins when compared with surface treatment. The
performance of the insecticide-resistant strain Hikone-R was better th
an that of the wild genotype LS (Leiden Standard). The positive test r
esults obtained with all four chemicals showed that the P450 system of
Drosophila is capable of metabolizing these genotoxins into electroph
ilic intermediates.