PERFORMANCE OF CD3XCD19 BISPECIFIC MONOCLONAL-ANTIBODIES IN B-CELL MALIGNANCY

Bispecific monoclonal antibodies, with a dual specificity for tumor as sociated antigens on target cells and for surface markers on immune ef fector cells, have been shown (in vitro) to be effective in directing and triggering effector cells to kill target cells resulting in target cell lysis. Bispecific monoclonal antibodies (BsAb) against the CD3 a ntigen on T cells and the CD19 antigen on B cell were developed. Data obtained by in vitro experiments might indicate that clinical response s in BsAb immunotherapy, will only be obtained in patients with minima l tumor load and may need additional T cell stimulation via cytokines such as IL-2. Although these experiments have shown us their limitatio ns, they also include the promise of BsAb-directed immunotherapy in B cell malignancy as further demonstrated during a Phase I trail, showin g little toxicity. Clearly, much remains to be done before this BsAb i s routinely used for therapy, but, the results presented show that the CD3xCD19 BsAb has a potential as a therapeutic agent in B cell malign ancy. This report describes the experiments performed to test a new im munotherapeutic approach for the treatment of B cell malignancy. Bispe cific antibodies are described that can target cytotoxic T cells to tu mor cells and elicit a cytolytic action towards these cancer cells.