BIOLOGICAL HETEROGENEITY OF DIFFUSE MIXED SMALL AND LARGE-CELL NON-HODGKINS-LYMPHOMAS ASSESSED BY DNA FLOW-CYTOMETRY AND KI67

Citation
G. Palestro et al., BIOLOGICAL HETEROGENEITY OF DIFFUSE MIXED SMALL AND LARGE-CELL NON-HODGKINS-LYMPHOMAS ASSESSED BY DNA FLOW-CYTOMETRY AND KI67, Leukemia & lymphoma, 19(5-6), 1995, pp. 467-472
Citations number
NO
Categorie Soggetti
Hematology
Journal title
ISSN journal
10428194
Volume
19
Issue
5-6
Year of publication
1995
Pages
467 - 472
Database
ISI
SICI code
1042-8194(1995)19:5-6<467:BHODMS>2.0.ZU;2-#
Abstract
The cell proliferative activity of the clinico-pathologically heteroge neous non-Hodgkin's lymphomas (NHL) included in the intermediate grade F category of the Working Formulation (WF) was investigated. S-phase fraction with flow cytometry on cell suspensions, and Ki67 on frozen t issue sections were performed in 42 F NHL. An avidin-biotin immunocomp lex method was used and 1000 cells from 10 representative fields were counted. DNA content, S-phase and Ki67 were also detected in 194 NHL c overing the whole spectrum of the WF. DNA content anomalies were found in 52 of 194 NHL. Their incidence, like that of S-phase fraction and Ki67 positive cells, progressively increased from low- to high-grade. A linear correlation was found between Ki67 and S-phase (r = .59). Usi ng the median value of proliferating cells obtained with both procedur es as a cut off, two very different groups of lymphomas could be disti nguished within a series of 42 F-intermediate NHL: with low and high p roliferative cell activity (p < .0001) that were termed F-low and F-hi gh, respectively. A intermediate group was placed between them. It dif fered significantly from the others if Ki67 was used but only from the F-high group if the S-phase fraction analysis was applied. No signifi cant differences were seen when comparing F-low with the single catego ries of low-grade NHL and F-high with H high-grade NHL; no significant differences were found between F-high and G, and between G and H cate gories. The existence of distinct groups of NHL in the F category, as defined by biological parameters assessing the cell proliferative acti vity, indicates that this category includes biologically heterogeneous lymphoma subtypes with different grades of aggressiveness. The result s also indicate that the G intermediate category displays proliferatio n indices similar to those of H high grade category.