Yh. Chuang et al., DIFFERENT KINDS OF ANTIGEN-PRESENTING CELLS EXERT DIFFERENT EFFECTS ON T-HELPER CELLS DEVELOPMENT, International archives of allergy and immunology, 111(4), 1996, pp. 366-371
It has been well documented that environmental factors such as antigen
-presenting cells and related cytokines can affect the development of
T-helper cells. The purpose of this study was to investigate the role
of different antigen-presenting cells on T-cell development. Ovalbumin
(OVA) combined with complete Freud's adjuvant (CFA) was used to sensi
tize mice subcutaneously or intraperitoneally, and then to follow-up p
roduction of IgG and IgE anti-OVA antibodies. In addition, semiquantit
ative PCR was used to determine the level of cytokine mRNA of differen
t antigen-presenting cells. Resulting data showed that antigen-present
ing cells expressed with different characteristics: (1) IgG2a anti-OVA
antibody was higher in mice sensitized subcutaneously compared to tho
se sensitized intraperitoneally. (2) The levels of cytokine mRNA were
higher in antigen-stimulated spleen cells of mice immunized subcutaneo
usly compared to those of mice immunized intraperitoneally. (3) Langer
hans cells expressed a high level of IL-12; in contrast, peritoneal B
cells expressed a high level of IL-10, but not IL-12. In summary, cyto
kine levels such as IL-IO and IL-12 were different among different kin
ds of APC, and their role in production of different isotypes of antib
odies needs further elucidation.