AN ENZYME-CONTAINING INK FOR SCREEN-PRINTED GLUCOSE SENSORS

A glucose sensor employing ferrocene-bound glucose oxidase was fabrica ted by the screen printing technique. In order to obtain high COD acti vity, glucose oxidase (GOD) was modified with ferrocenecarboxylic acid (fc) as an electron mediator. Simultaneous spectroscopic and electroc hemical analyses showed that ferrocene-bound GOD (fc-GOD) displayed an increase in catalytic current. The ferrocene acts as an electron tran sfer relay between the active center of the enzyme and the gold electr ode. The screen printing process was used to print successfully the un ique fc-GOD containing ink onto the gold electrode patterned on the gl ass plate. The fc-GOD containing ink was specially developed to be sui table for the printing apparatus. The printed glucose sensor was shown to offer a stable calibration profile and stable electrochemical prop erties.