ENDOGENOUS IL-10 PROTECTS MICE FROM DEATH DURING SEPTIC PERITONITIS

IL-10 production during endotoxic shock is part of a protective mechan ism that involves IL-10-induced inhibition of TNF synthesis. We sought to determine the role of IL-10 in septic peritonitis induced by cecal ligation and puncture (CLP). CLP led to a rapid induction of IL-10 mR NA in various organs of C57Bl/6 mice. Ih liver, IL-10 mRNA was detecta ble within 1 h following CLP, while in spleen and lungs, IL-10 mRNA wa s detected from 2 to 4 h and onward. IL-10 protein became detectable i n plasma 2 h after CLP, reaching peak concentrations after 12 h (12.7 +/- 5.7 ng/ml). Pretreatment (-2 h) with anti-IL-l0 mAb resulted in hi gher plasma TNF levels following CLP when compared with mice treated w ith control mAb. Plasma IL-l activity and IFN-gamma remained undetecta ble in virtually all mice. Anti-IL-10 enhanced mortality after CLP (p < 0.05 by log-rank test). Addition of anti-TNF mAb did not influence t he increased mortality associated with anti-IL-10 treatment. Septic pe ritonitis is associated with sustained production of IL-10 in various organs, which serves to protect the host against lethality.