E. Roca et al., CONTINUOUS ETHANOLIC FERMENTATION BY SACCHAROMYCES-CEREVISIAE IMMOBILIZED IN CA-ALGINATE BEADS HARDENED WITH AL3+, Biotechnology techniques, 9(11), 1995, pp. 815-820
The most suitable conditions for the hardening of alginate bioparticle
s by Al3+ obtained were: Al3+, 0.3 M; time of treatment 5 min and yeas
t concentration in the gel, 3 g dry weight/L. The hardened biocatalyst
was used to carry out an ethanolic fermentation process in a semi-pil
ot packed-bed reactor and no breakage of the bioparticles was observed
, although the lower part beads had a flat shape, which caused a 11% d
ecrease in bed height. Ethanol productivities up to 31 g/L . h were ob
tained.