Polyionic hydrogels have been prepared by complexation of chitosan and
xanthan. These hydrogels have been used to immobilize lipase from por
cine pancreas (E.C. 3.1.1.3). Immobilization efficiency varied between
90 and 99% of initial activity. Immobilized lipase retained its activ
ity towards hydrolysis of olive oil in water as an emulsion and of oli
ve oil in isooctane.