THE 9-KDA CALBINDIN GENE OF ROUSETTUS-AEGYPTIACUS - ITS IDENTIFICATION AND ISOLATION FROM A GENOMIC LIBRARY

A genomic library of the fruit bat (Rousettus aegyptiacus) was constru cted in lambda phage gt11. The titre of the library was determined to be 2 x 10(5) pfu/ml. The genomic library was amplified and the titre o f the amplified library increased 300-fold to 7 x 10(7) pfu/ml. The li brary was screened by in situ hybridization techniques using a fragmen t of the mouse 9-kDa calbindin cDNA as a probe. Screening of 10(5) pla ques yielded a positive clone. Three additional rounds of screening we re performed to purify the positive. Lambda phage DNA was isolated fro m the positive clone and restriction digest analysis, followed by hybr idization studies, was performed on these digests in order to determin e the location of the bat 9-kDa calbindin gene in the insert of the la mbda phage vector. Restriction maps so derived were interpreted from t he published sequence for the rat 9-kDa calbindin gene and indicate th e successful isolation of the 9-kDa calbindin gene of Rousettus aegypt iacus.