K. Takayama et al., DEFECTS IN THE DNA-REPAIR AND TRANSCRIPTION GENE ERCC2 IN THE CANCER-PRONE DISORDER XERODERMA-PIGMENTOSUM GROUP-D, Cancer research, 55(23), 1995, pp. 5656-5663
Xeroderma pigmentosum (XP) is a sun-sensitive, cancer-prone genetic di
sorder characterized by a defect in nucleotide excision repair. The hu
man nucleotide excision repair and transcription gene ERCC2 is able to
restore survival to normal levels after exposure to UV light in XP co
mplementation group D cells. No enhancement of UV survival is seen in
groups C, E, F, or G. XP-CS-2 cells are complemented by ERCC2, confirm
ing the reassignment to group D of this combined XP/Cockayne's syndrom
e patient. Nucleotide sequence analysis of the ERCC2 cDNA from five XP
group D cell strains [XP6BE(SV40), XP17PV, XP102LO, A31-27 (a HeLa/XP
1O2LO hybrid), and XP-CS-2] revealed mutations predominantly affecting
previously identified functional domains. The mutations include base
substitutions resulting in amino acid substitutions, deletions due to
splicing alterations, and defects in expression, XP6BE(SV40), XP17PV,
XP102LO, and A31-27 all have one allele with an Arg683 to Trp substitu
tion within the putative nuclear location signal. The genetic disorder
trichothiodystrophy (which is not cancer-prone) can also result from
mutations in the ERCC2 gene, some of which are the same as those found
in XP-D. The various clinical presentations can be correlated with th
e particular mutations found in the ERCC2 locus.