ASCORBIC ACID-2-O-BETA-GLUCURONIDE, A NEW METABOLITE OF VITAMIN-C IDENTIFIED IN HUMAN URINE AND UREMIC PLASMA

Authors
GALLICE P SARRAZIN F POLVERELLI M CADET J BERLAND Y CREVAT A
Citation
P. Gallice et al., ASCORBIC ACID-2-O-BETA-GLUCURONIDE, A NEW METABOLITE OF VITAMIN-C IDENTIFIED IN HUMAN URINE AND UREMIC PLASMA, Biochimica et biophysica acta (G). General subjects, 1199(3), 1994, pp. 305-310
Citations number
16
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta (G). General subjects → ACNP
ISSN journal
03044165
Volume
1199
Issue
3
Year of publication
1994
Pages
305 - 310
Database
ISI
SICI code
0304-4165(1994)1199:3<305:AAANMO>2.0.ZU;2-S
Abstract
A new metabolite of ascorbic acid has been isolated by a multi-step ch romatographic procedure both from normal human urine and uremic plasma . Nuclear Magnetic Resonance studies, and chemical and enzymic analyse s indicated that the compound is a conjugated structure consisting of equimolar ascorbic and beta-D-glucuronic acids. We determined the pK(a ) value of the ascorbic acid moiety of the compound on the basis of va riations of ultraviolet absorbances as a function of pH. Results showe d that glucuronic acid is coupled to the 2-position of ascorbic acid.