D. Leppert et al., STIMULATION OF MATRIX METALLOPROTEINASE-DEPENDENT MIGRATION OF T-CELLS BY EICOSANOIDS, The FASEB journal, 9(14), 1995, pp. 1473-1481
Prostaglandin E(2) (PGE(2)) and leukotriene B4 (LTB4), at nanomolar to
micromolar concentrations, elicited migration of human blood T cells
and cultured T lymphoblastoma cells of the Tsup-1 line through a layer
of Matrigel basement membrane matrix, The density of Tsup-1 cell high
-affinity receptors was low for PGE(2) and high for LTB4, resulting in
respectively predominant chemokinetic and chemotactic: stimulation of
migration, Migration-enhancing concentrations of PGE(2) and LTB4 also
increased Tsup-1 cell content and secretion of matrix metalloproteina
ses (MMPs) 2, 3, and 9, which were quantified by Western blots and zym
ography, and augmented Tsup-1 cell-surface expression of the MMPs, as
shown by flow cytometry, That a specific MMP inhibitor suppressed migr
ation of blood T cells and Tsup-1 cells through Matrigel, but did not
affect PGE(2)- and LTB4-initiated T cell migration through micropore f
ilters without Matrigel, suggests dual requirements for MMP expression
and enhanced motility in T cell passage through basement membranes.