GLYCEROL AS A NEW DISSOLUTION MEDIUM FOR ALPHA-CYCLODEXTRIN, BETA-CYCLODEXTRIN AND GAMMA-CYCLODEXTRIN FOR PREPARING STEREOSELECTIVE STATIONARY PHASES FOR GAS-LIQUID-CHROMATOGRAPHY

Glycerol was applied as the dissolution medium for alpha, beta- and ga mma-cyclodextrins and its gas-liquid chromatographic performance was c ompared with that of formamide. Various stationary glycerol-based chir al phases for classical gas-liquid chromatography were examined. The c olumns containing glycerol appeared relatively stable in comparison wi th those prepared with formamide. Glycerol-based phases functioned ove r the temperature ranged 80-100 degrees C for about 400 h without any changes indicating losses of glycerol or activity. Owing to the lower stability of cyclodextrin complexes in glycerol medium relative to tho se in formamide, and the fact that glycerol-based columns allow operat ion ca. 40 degrees C higher, many separations which were previously un reported at higher temperatures were achieved. The model compounds tes ted were (+/-)-alpha-pinene, cis/trans-decalin, cis/trans-anethole, ci s-trans-isosafrol, cis-trans-isoeugenol, (+/-)-camphor, (+/-)-fenchone , (+/-)-isomenthone, (+/-)-isopinocampheol, (+/-)-borneol and (+/-)-is oborneol.