INDUCTION OF URINARY PLASMINOGEN-ACTIVATOR BY RETINOIC ACID RESULTS IN INCREASED INVASIVENESS OF HUMAN PROSTATE-CANCER CELLS PC-3

Authors
Citation
Df. Liu et Sa. Rabbani, INDUCTION OF URINARY PLASMINOGEN-ACTIVATOR BY RETINOIC ACID RESULTS IN INCREASED INVASIVENESS OF HUMAN PROSTATE-CANCER CELLS PC-3, The Prostate, 27(5), 1995, pp. 269-276
Citations number
34
Categorie Soggetti
Endocrynology & Metabolism","Urology & Nephrology
Journal title
ISSN journal
02704137
Volume
27
Issue
5
Year of publication
1995
Pages
269 - 276
Database
ISI
SICI code
0270-4137(1995)27:5<269:IOUPBR>2.0.ZU;2-7
Abstract
Overproduction of uPA by prostate cancer cells in vivo results in tumo r invasiveness and osteoblastic skeletal metastasis due to its mitogen ic actions in osteoblasts. In the present study we have examined the e ffect of several growth factors and steroid hormones on regulating uPA gene expression in the human prostate cancer cell line (PC-3). Treatm ent of these cells with dexamethasone (Dex) caused a decrease, whereas epidermal growth factor (EGF) and fetal bovine serum (FBS) increased uPA expression in a dose-dependent manner. Trans retinoic acid (RA) al so induced uPA mRNA and protein production in a dose-dependent manner (10(-6) to 10(-9) M). This increase was seen as early as 2 hr of treat ment until 48 hr. Dex treatment resulted in decreased tumor cells inva siveness, whereas exposure to EGF and RA caused an increase in the inv asive capacity of PC-3 cells. These studies should help to better unde rstand the control mechanism of uPA expression in prostate cancer, whe re uPA has been implicated as a major pathogenetic factor. (C) 1995 Wi ley-Liss, Inc.