Vh. Bustamante et al., IDENTIFICATION OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI USING THE RPOB GENE AND A CRYPTIC DNA FRAGMENT FROM C-JEJUNI, Gene, 165(1), 1995, pp. 1-8
Campylobacter jejuni (Cj) and C. coli (Cc) clinical isolates, obtained
from three different sources, were characterized using two Cj DNA pro
bes, CJ01 and CJ02. These probes were selected at random by virtue of
their stability in Escherichia coli (Ec). CJ01 hybridized specifically
with DNA from Cj reference strains, but not with DNA from Cc, C. lari
(Cl) nor C. fetus (Cf) reference strains. Using clinical isolates cha
racterized by genome-genome hybridization and biotype, CJ01 hybridized
with DNA derived from all Cj strains. However, DNA from four out of t
en Cc strains, from three different sources, also hybridized with CJ01
, suggestive of this region being heterogeneous between clinical isola
tes of both species. The nucleotide sequence analysis of CJ01 reveals
two incomplete open reading frames (ORFs) that did not show significan
t homology with any other known sequences. CJ02 hybridized specificall
y with DNA from Cj and Cc reference strains, but not with DNA from CI
and Cf reference strains. The specificity and sensitivity were maintai
ned upon hybridization with DNA from 31 clinical isolates. CJ02 has an
uninterrupted ORF whose deduced amino-acid sequence showed extensive
homology with the central region of the Ec and Salmonella typhimurium
(St) RNA polymerase beta subunits (52 and 66% similarity, respectively
). The most conserved segments correspond to putative functional domai
ns.