GREEN FLUORESCENT PROTEIN-PRODUCTION IN THE CELLULAR SLIME-MOLDS POLYSPHONDYLIUM-PALLIDUM AND DICTYOSTELIUM-DISCOIDEUM

Authors
FEY P COMPTON K COX EC
Citation
P. Fey et al., GREEN FLUORESCENT PROTEIN-PRODUCTION IN THE CELLULAR SLIME-MOLDS POLYSPHONDYLIUM-PALLIDUM AND DICTYOSTELIUM-DISCOIDEUM, Gene, 165(1), 1995, pp. 127-130
Citations number
7
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
165
Issue
1
Year of publication
1995
Pages
127 - 130
Database
ISI
SICI code
0378-1119(1995)165:1<127:GFPITC>2.0.ZU;2-B
Abstract
The green fluorescent protein-encoding gene from Aequorea victoria has been eloped into several different transforming vectors and expressed in the cellular slime molds, Polysphondylium pallidum and Dictyosteli um discoideum. We find that the protein is stable and non-toxic in bot h species, can be easily visualized in living and fixed specimens, and can be used to purify rare cells by fluorescence-activated cell sorti ng (FAGS).