Jp. Skvorak et al., ENDOTHELIN ACTS AS A PARACRINE REGULATOR OF STRETCH-INDUCED ATRIAL-NATRIURETIC-PEPTIDE RELEASE, American journal of physiology. Regulatory, integrative and comparative physiology, 38(5), 1995, pp. 1093-1098
Several lines of evidence suggest a paracrine regulatory role for endo
thelin (ET) in the release of atrial natriuretic peptide (ANP). To inv
estigate this possibility, we used the ET A-type receptor (ET(A)-R) co
mpetitive inhibitor cyclo(D-Asp-Pro-D-Val-Leu-D-Trp) (BQ-123) in isola
ted perfused atria to determine the effect of endogenously produced ET
on the release of ANP. Initially, we found that high pressure (8-10 m
mHg) increased the mean ANP secretion rate by 117.3 +/- 21.2% (P < 0.0
5). Next, we found that at high pressure 50 nM of exogenously applied
ET significantly augmented the stretch-induced release of ANP (P < 0.0
5) and that this response could be significantly attenuated in a dose-
dependent manner by 1 and 3 mu M BQ-123 (P < 0.05). These experiments
proved the efficacy of the inhibitor in our model. Subsequently, we fo
und that the stretch-induced release of ANP was significantly reduced
to 51.5 +/- 13.0 and 22.3 +/- 11.8% by 1 and 3 mu M BQ-123, respective
ly (P < 0.05). Because the perfused atria model eliminates systemic ca
rdiovascular effects, allows control and direct recording of the intra
-atrial pressure, and preserves the potential endothelium-myocyte cont
rol system, we conclude that the stretch-induced release of ANP is par
tially regulated by ET and that the ET is locally produced and constit
utes a paracrine control system.