DETERMINATION OF INOSITOL PHOSPHATES IP3-IP6 IN RAPESEED AND RAPESEEDMEAL BY AN HPLC METHOD .2. INVESTIGATIONS OF RAPESEED AND RAPESEED MEAL AND COMPARISON WITH OTHER METHODS
B. Matthaus et al., DETERMINATION OF INOSITOL PHOSPHATES IP3-IP6 IN RAPESEED AND RAPESEEDMEAL BY AN HPLC METHOD .2. INVESTIGATIONS OF RAPESEED AND RAPESEED MEAL AND COMPARISON WITH OTHER METHODS, Fett, 97(10), 1995, pp. 372-374
Because of their structure, phytic acid and its degradation products a
re able to bind other feedstuff ingredients, such as proteins, starch,
cations or enzymes. This ability depends on the grade of phosphorylat
ion. In order to investigate the influence of industrial processing on
the inositol phosphate composition of rapeseed, 17 rapeseed samples a
nd 8 rapeseed meal samples were analysed by an HPLC method. Only a sma
ll grade of degradation of IP6 was noticed both in rapeseed meal, wher
e also IP4 could be determined, and in rapeseed. The results were comp
ared with two other phytic acid determination methods. The precipitati
on method with FeCl3 of Wheeler and Ferrel and a photometric method we
re used for this comparison, both methods for determination of the sum
of inositol phosphates. The results of the HPLC method and the method
of Wheeler and Ferrel correspond in most cases, but the values or the
photometric method are far higher.