A. Guttman et T. Pritchett, CAPILLARY GEL-ELECTROPHORESIS SEPARATION OF HIGH-MANNOSE TYPE OLIGOSACCHARIDES DERIVATIZED BY 1-AMINOPYRENE-3,6,8-TRISULFONIC ACID, Electrophoresis, 16(10), 1995, pp. 1906-1911
In this paper we report the capillary gel electrophoresis separation o
f 1-aminopyrene-3,6,8-trisulfonic acid (APTS) labeled oligosaccharides
, released enzymatically from bovine pancreatic ribonuclease B. The re
leased and labeled high-mannose structures were identified by spiking
the separated peaks with the appropriate commercially available indivi
dual oligosaccharides. Baseline separation of the three positional iso
mers of the mannose-7 and mannose-8 oligosaccharides was attained. Com
parison of the electrophoretic mobilities of the high-mannose type bra
nched carbohydrates to the linear molecules of maltooligosaccharides (
glucose oligomers) have been shown using different gel concentrations
in the running buffer system. We observed that increasing gel concentr
ation in the running buffer causes an increase in the relative mobilit
y values of the high-mannose type carbohydrate molecules compared to t
he linear glucose oligomers. Analysis of our data indicated that this
increase in relative migration time was not due to sieving, but seemed
to be related to the mannose content and hydrodynamic volume of the b
ranched glycans as well as to the viscosity of the separation medium.