ART UPDATED 2-DIMENSIONAL GEL DATABASE OF RAT-LIVER PROTEINS USEFUL IN GENE-REGULATION AND DRUG EFFECT STUDIES

Authors
ANDERSON NL ESQUERBLASCO R HOFMANN JP MEHEUS L RAYMACKERS J STEINER S WITZMANN F ANDERSON NG
Citation
Nl. Anderson et al., ART UPDATED 2-DIMENSIONAL GEL DATABASE OF RAT-LIVER PROTEINS USEFUL IN GENE-REGULATION AND DRUG EFFECT STUDIES, Electrophoresis, 16(10), 1995, pp. 1977-1981
Citations number
10
Categorie Soggetti
Biochemical Research Methods
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
16
Issue
10
Year of publication
1995
Pages
1977 - 1981
Database
ISI
SICI code
0173-0835(1995)16:10<1977:AU2GDO>2.0.ZU;2-I
Abstract
We have improved upon the reference two-dimensional (2-D) electrophore tic map of rat liver proteins originally published in 1991 (N. L. Ande rson et al., Electrophoresis 1991, 12, 907-930). A total of 53 protein s (102 spots) are now identified, many by microsequencing. In most cas es, spots cut from wet, Coomassie Blue stained '2-D gels were submitte d to internal tryptic digestion [2], and individual peptides, separate d by high-performance liquid chromatography (HPLC), were sequenced usi ng a Perkin-Elmer 477A sequenator. Additional spots were identified us ing specific antibodies.