A NOVEL CHEMOENZYMATIC GLYCOSYLATION STRATEGY - APPLICATION TO LYSOZYME MODIFICATION

Hen egg lysozyme has been non-specifically glycosylated using a novel two-step strategy, First, a number of sucrose molecules have been chem ically bound to the protein surface lysines, then the glycosidic chain s have been enzymically lengthened, using a glycosyltransferase. For t his task, a fructosyltransferase and a levansucrase have been tested, the latter appearing as the most effective one, In all cases, reaction s have been optimised and several degrees of modification have been ob tained, Finally, the effects of the modifications on lysozyme hydropho bicity, hydrolytic activity, hydrolysis substrate affinity and thermos tability have been assessed.