THE KINETICS OF RCC1 INCLUSION-BODY FORMATION IN ESCHERICHIA-COLI

The Regulator of Chromosome Condensation protein (RCC1) is located in both the soluble and inclusion body (IB) fractions of the whole cell l ysate when expressed in Escherichia coli BL21 (pLysS) at temperatures below 30 degrees C. When bacterial growth was carried out at 20 degree s C, the majority of the RCC1 remained soluble up to 5.5 h postinducti on. When the temperature was raised to 25 degrees C, RCC1 IB was domin ant by 1.5 h postinduction. The shift in RCC1 IB formation with temper ature suggests that in addition to increased translation rates, foldin g and aggregation processes may contribute to RCC1 IB formation at hig her temperatures. (C) 1995 John Wiley & Sons, Inc.