C. Oesterreicher et al., HBV AND HCV GENOME IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS IN PATIENTS UNDERGOING CHRONIC-HEMODIALYSIS, Kidney international, 48(6), 1995, pp. 1967-1971
Patients undergoing chronic hemodialysis are at risk for infection wit
h hepatitis B virus (HBV) and hepatitis C virus (HCV). As peripheral b
lood mononuclear cells (PMNC) are known to be susceptible to infection
of both HBV and HCV, assessment of viral genomes in those cells could
uncover occult infections not detected by serologic methods or virus
determination in serum. We investigated all 67 patients undergoing chr
onic hemodialysis at a single dialysis unit by PCR for the presence of
HBV or HCV genomes in serum as well as in PMNC. None of the 67 patien
ts was HBsAg positive or showed HBV-DNA in serum, but in 5 patients HB
V-DNA in PMNC was detected as the only marker of HBV-infection; those
patients were also anti-HBc negative. In 9 patients HCV-RNA was positi
ve in serum; in 5 of those patients it was also found in PMNC. Three o
f these infected patients were negative for anti-HCV. One other patien
t had no anti-HCV or HCV-RNA in serum, but was positive for HCV-RNA in
PMNC. Thus, in 6 patients (8.9%) undergoing chronic hemodialysis we f
ound evidence of infection with HBV or HCV by detecting Viral genomes
in PMNC without the presence of viremia, antigenemia or specific viral
antibodies in serum. The detection of viral genomes in PMNC could be
useful in the positive identification of additional potentially infect
ious patients.