YEAST GLOBAL TRANSCRIPTIONAL REGULATORS SIN4 AND RGR1 ARE COMPONENTS OF MEDIATOR COMPLEX RNA-POLYMERASE-II HOLOENZYME

Authors
LI Y BJORKLUND S JIANG YW KIM YJ LANE WS STILLMAN DJ KORNBERG RD
Citation
Y. Li et al., YEAST GLOBAL TRANSCRIPTIONAL REGULATORS SIN4 AND RGR1 ARE COMPONENTS OF MEDIATOR COMPLEX RNA-POLYMERASE-II HOLOENZYME, Proceedings of the National Academy of Sciences of the United Statesof America, 92(24), 1995, pp. 10864-10868
Citations number
27
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
92
Issue
24
Year of publication
1995
Pages
10864 - 10868
Database
ISI
SICI code
0027-8424(1995)92:24<10864:YGTRSA>2.0.ZU;2-1
Abstract
Sin4 and Rgr1 proteins, previously shown by genetic studies to play bo th positive and negative roles in the transcriptional regulation of ma ny genes, are identified here as components of mediator and RNA polyme rase II holoenzyme complexes. Results with Sin4 deletion and Rgr1 trun cation strains indicate the association of these proteins in a subcomp lex comprising Sin4, Rgr1, Gal11, and a 50-kDa polypeptide. Taken toge ther with the previous genetic evidence, our findings point to a role of the mediator in repression as well as in transcriptional activation .