CHROMOSOMAL LOCALIZATION OF GENES REQUIRED FOR THE TERMINAL STEPS OF OXIDATIVE-METABOLISM - ALPHA-SUBUNITS AND GAMMA-SUBUNITS OF ATP SYNTHASE AND THE PHOSPHATE CARRIER

The terminal steps of oxidative phosphorylation include transport of p hosphate and ADP into the mitochondrial matrix, synthesis of ATP in th e matrix, and transport of the product ATP into the cytosol where it c an be utilized to perform cellular work. Three nuclear genome encoded membrane proteins, namely, the phosphate carrier (PHC), the adenine nu cleotide carrier (ANT), and the ATP synthase complex, consisting of at least 13 individual subunits, catalyze these reactions. The locations of the alpha and gamma subunits of the mitochondrial ATP synthase com plex and the mitochondrial phosphate carrier, PHC, on human chromosome s were determined using cloned rat liver cDNA as probes. Human homolog ues of the ex subunit are on chromosomes 9 and 18, the gamma subunit a re on chromosomes 10 and 14, and the PHC was localized to chromosome 1 2.