MOLECULAR-CLONING OF ALPHA(1D)-ADRENERGIC RECEPTOR AND TISSUE DISTRIBUTION OF 3 ALPHA(1)-ADRENERGIC RECEPTOR SUBTYPES IN MOUSE

A partial cDNA encoding most of the third intracellular loop of the mo use alpha(1d)-adrenergic receptor subtype was amplified from hippocamp us by reverse transcription-polymerase chain reaction (RT-PCR) using d egenerate oligodeoxynucleotide primers. This DNA fragment was used as a probe to isolate an alpha(1d)-adrenergic receptor cDNA from a mouse brain cDNA library. The deduced amino acid sequence encodes a potentia l protein of 562 amino acids, and northern hybridization of poly(A)(+) RNA isolated from mouse brain detected a single 3.0-kb transcript, Pa rtial cDNA fragments of the alpha(1b)-and alpha(1a)-adrenergic recepto r subtypes were also amplified from mouse brain and sequenced. Analysi s of the mRNA expression by RT-PCR indicated that the alpha(1)-adrener gic receptors are widely distributed in mouse tissues. The alpha(1d) s ubtype is expressed in brain areas such as hippocampus, striatum, and brainstem and also in many extracerebral tissues, such as lung, liver, heart, kidney, and spleen. The alpha(1a) subtype is also expressed in many tissues, whereas the alpha(1b) subtype has a more restricted exp ression, with high levels in striatum, brainstem, and diencephalus.