ULTRASTRUCTURAL CYTOCHEMISTRY OF HUMAN GASTRIC-CANCER - ELECTRON-MICROSCOPIC OBSERVATIONS OF 5 ORGANELLAE MARKER ENZYMES

The distribution of ALPase, ACPase, G6Pase TPPase and CCOase of gastri c cancer and normal gastric epithelium were studied ultrastructurally, The results showed that normal gastric epithelium had no ALPase react ion, The reactions of ACPase, G6Pase, TPPase and CCOase were found in the corresponding organellae which were cosistent with their functions , In tubular adenocarcinoma cells, their reactions were more apparent in the corresponding organellae, Some cells of tubular adenocarcinomas showed ALPase reaction. The mucinous adenocarcinoma cells had higher ACPase and TPPase reactions. In poorly differentiated adenocarcinoma c ells, the five marker enzymes showed negative or faint reactions. The biological significance and mechanisms of distribution of the five mar ker enzymes were discussed.