Y. Yong et Jl. Dreyer, DISTRIBUTION OF 6 TRANSPLASMA MEMBRANE NADH-DEHYDROGENASES IN RAT-BRAIN TISSUE, Developmental brain research, 89(2), 1995, pp. 235-252
Transplasma membrane redox plays a significant role in cellular activa
tion and growth. Six isoenzymes could be prepared from purified rat br
ain synaptic plasma membrane. Polyclonal antibodies have been prepared
against six transplasma membrane oxydoreductases (PMO-I to PMO-VI) an
d the tissue distribution of the various iso-enzymes have been investi
gated in adult rat brains by means of immunohistochemistry. PMO-I is d
ensely observed in layers I, TV and V of the parietal cortex, in CA1 o
f the hippocampus (except for the molecular layer), in the caudate put
amen, in the dorsal, granular and ventral parts of the auditory nuclei
, in some loci of the vestibular nuclei as well as in the deep cerebel
lar nucleus and in the granular layer of the cerebellar cortex. PMO-II
is mainly located in the polymorphic layer of the dendate gyrus and i
n the deep cerebellar nucleus and in the granular layer of the cerebel
lar cortex. PMO-III is abundant in the piriform cortex, in the pyramid
al layers of both CA1 and CA2, in the diagonal band of the basal gangl
ia, in the supraoptic nucleus and in various loci of the magnetocellul
ar paraventricular nucleus of the hippothalamus as well as in the vest
ibular nuclei from the brain stem. In addition PMO-III is also densely
present in motor nuclei (oculomotor, facial, hypoglossal and ambiguus
nuclei), in the reticular formation and in the deep cerebellar nucleu
s as well as in the Purkinje layer of the cerebellar cortex. PMO-IV ha
s a similar location but is less abundant in the vestibular nuclei of
the sensory brain stem and in the motor nucleus. PMO-V in contrast is
poorly present in most brain areas compared to the other iso-enzymes,
apart of the Purkinje layer of the cerebellar cortex. Finally PMO-VI i
s mainly present in the oriens layer and in the stratum radiatum of th
e hippocampus formation, in the supraoptic and lateral magnocellular n
ucleus of the hypothalamus, in the mesencephalic trigeminal nucleus, i
n the ventral auditory nucleus and in the facial nucleus of the brain
stem as well as in red nucleus of the reticular formation and in the P
urkinje layer of the cerebellar cortex. These data show that the iso-e
nzymes are located in specific brain nuclei. The significance of the r
esults in respect to the yet very poorly defined function of PMO's is
discussed.