THE DEVELOPMENT AND VALIDATION OF A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY (HPLC) TANDEM MASS-SPECTROMETRY ASSAY FOR FENTICONAZOLE IN HUMANPLASMA AND COMPARISON WITH AN HPLC-UV METHOD

Authors
SPEED W LONG JM SIMMONDS RJ ENOS TA
Citation
W. Speed et al., THE DEVELOPMENT AND VALIDATION OF A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY (HPLC) TANDEM MASS-SPECTROMETRY ASSAY FOR FENTICONAZOLE IN HUMANPLASMA AND COMPARISON WITH AN HPLC-UV METHOD, Rapid communications in mass spectrometry, 9(14), 1995, pp. 1452-1456
Citations number
1
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
Journal title
Rapid communications in mass spectrometry → ACNP
ISSN journal
09514198
Volume
9
Issue
14
Year of publication
1995
Pages
1452 - 1456
Database
ISI
SICI code
0951-4198(1995)9:14<1452:TDAVOA>2.0.ZU;2-I
Abstract
A method is described for the determination of fenticonazole in human female plasma. The method utilizes high performance liquid chromatogra phy coupled to atmospheric pressure positive-ion chemical ionization t riple quadrupole mass spectrometry. Multiple reaction monitoring is em ployed for selectivity and sensitivity which enables quantification ov er the range 0.5-20 ng mL(-1) with acceptable precision and accuracy. A comparison is made with an existing HPLC-W assay and the utility of the technology of combined liquid chromatography and tandem mass spect rometry for subnanogram per mt assays is discussed.